Molecular Biology Insights Oligo v7.57





OLIGO Primer Analysis Software is the essential tool for designing and

analyzing sequencing and PCR primers, synthetic genes, and various kinds of

probes including siRNA and molecular beacons.

Based on the most up-to date nearest neighbor thermodynamic data, Oligo's

search algorithms find optimal primers for PCR, including TaqMan, highly

multiplexed, consensus or degenerate primers. Multiple file batch

processing is possible. It is also an invaluable tool for site directed

mutagenesis.

For each primer or primer pair, Oligo's various analysis windows show a

multitude of useful data, such as DNA and RNA secondary structure, dimer

formation, false priming and homology, internal stability, composition and

physical properties. With Oligo you can analyze open reading frames down to

predicted molecular weight and pKa of proteins, and search for restriction

enzyme sites, not only in DNA but also in reverse-translated proteins.

The first version of the software appeared on the market in 1989. It went

through several modifications, and the last one, the change from version 6

to 7, was the most comprehensive. Oligo 7 can automatically select multiplex

primers, process sequence files in batch modes, automatically design PCR

primers to cover multiple DNA regions in just one search and automatically

find primer/probe sets for real time PCR or finds nested primers sets. Oligo

search protocols (scoring system) may be customized in detail, so you may

optimize the results according to your specific needs.


OLIGO's comprehensive functionality matches several other commercial primer design applications put together, so you can design probes for high throughput analysis, multiplex them or focus on one set of primers/probes and optimize it for a new, not yet published application.


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